Recombinant XCR1 antibody

Catalog No. ABIN7543269

Product Details anti-XCR1 Antibody

Target: XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This XCR1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), In vivo Studies (in vivo)

Purpose: Anti-XCR1 [MARX10], Rabbit IgG, kappa

Specificity: This antibody binds mouse XCR1, which is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80 % of CD8+ the dendritic cells. It transduces a signal by increasing the intracellular calcium ions level. This antibody is also reported to cross-reacts with rat CXR1.

Cross-Reactivity: Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 x 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat-inactivated B. pertussis.

Clone: MARX10

Isotype: IgG kappa

Application Details

Application Notes: This antibody was used to stain cryostat sections (12 μm) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20 % of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012, PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1⁺ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay, MARX10-OVA was found to be highly effective in inducing CD8⁺ T cell cytotoxicity in vivo (Hartung et al., 2015, PMID: 25520399).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for XCR1

Target: XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

Alternative Name: XCR1 (XCR1 Products)

Background: MXCR1, Ccxcr1, Chemokine XC receptor 1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1, MARX1O

UniProt: Q9R0M1