Recombinant HLA-A2 antibody

Catalog No. ABIN7543207

Product Details

Target: HLA-A2

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), ELISA

Purpose: Anti-HLA-A2 [BB7.2], Rabbit IgG, kappa

Specificity: This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.

Clone: BB7-2

Isotype: IgG kappa

Application Details

Application Notes: The binding specificity of BB7.2 towards HLA-A2 was assessed using complement-mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 Molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: HLA-A2

Target Type: Influenza Protein

Background: HLA-A, Human leukocyte antigen A, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A-2 HLA-A, HLA class I histocompatibility antigen A-28 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A-28

UniProt: P04439