Recombinant EGFR (Nimotuzumab Biosimilar) antibody
Reactivity: Human
BR, FACS, IHC
Host: Mouse
Chimeric
h-R3
unconjugated
Recombinant Antibody
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- Target See all EGFR (Nimotuzumab Biosimilar) products
- EGFR (Nimotuzumab Biosimilar)
- Antibody Type
- Recombinant Antibody
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Chimeric
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Conjugate
- This EGFR (Nimotuzumab Biosimilar) antibody is un-conjugated
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Application
- Blocking Reagent (BR), Flow Cytometry (FACS), Immunohistochemistry (IHC)
- Purpose
- Anti-EGFR domain III [h-R3 (Nimotuzumab)], Mouse IgG2a, kappa
- Specificity
- Binds to human EGFR domain III at a site distinct from antibody 7A7 (Ab00134) (Talavera et al. 2011)
- Characteristics
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Original Species of Ab: Human
Original Format of Ab: IgG1
- Purification
- Protein A affinity purified
- Immunogen
- This antibody we generated by humanisation of the murine antibody R3, raised against EGFR purified from placenta membranes and A431 tumors.
- Clone
- H-R3
- Isotype
- IgG2a kappa
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- Application Notes
- This antibody binds to human EGFR and inhibits EGF-dependent clonogenic growth of NRK cells and completely blocks EGF stimulated autophosphorylation of the receptor (Fernandez 1992)
- Comment
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This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- EGFR (Nimotuzumab Biosimilar)
- Abstract
- EGFR (Nimotuzumab Biosimilar) Products
- Target Type
- Biosimilar
- Background
- ERBB-D3, ERBB1, HER1, PIG61, mENA, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, epidermal growth factor
- UniProt
- P00533
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