Recombinant TNF alpha (Adalimumab Biosimilar) antibody
Reactivity: Human
ELISA, BR, IF, IHC
Host: Mouse
Chimeric
D2E7
unconjugated
Recombinant Antibody
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- Target See all TNF alpha (Adalimumab Biosimilar) products
- TNF alpha (Adalimumab Biosimilar)
- Antibody Type
- Recombinant Antibody
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Chimeric
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Conjugate
- This TNF alpha (Adalimumab Biosimilar) antibody is un-conjugated
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Application
- ELISA, Blocking Reagent (BR), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Purpose
- Anti-TNF alpha [D2E7 (Adalimumab)], Mouse IgG2a, kappa
- Specificity
- Binds to soluble TNF-alpha, but not to lymphotoxin (TNF beta).
- Cross-Reactivity
- Mouse
- Characteristics
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Original Species of Ab: Human
Original Format of Ab: IgG1
- Purification
- Protein A affinity purified
- Immunogen
- This antibody was generated by affinity maturation from the 2SD4 scFv, which in turn was selected on human TNF alpha by guided phage-display technology, using the murine anti-hTNF alpha antibody Mab32 as a template (c.f. also US Patent 6090382).
- Clone
- D2E7
- Isotype
- IgG2a kappa
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anti-TNF alpha (Adalimumab Biosimilar) antibody
Research Grade Reactivity: Human ELISA, FACS Host: Human Monoclonal unconjugated Recombinant Antibody
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- Application Notes
- Adalimumab binds specifically to TNF and neutralizes the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors. Adalimumab, unlike Infliximab (Ab00146), was shown to be cross-reactive with mouse TNF alpha (Arnoult 2017, PMID: 28584008).
- Comment
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This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- TNF alpha (Adalimumab Biosimilar)
- Abstract
- TNF alpha (Adalimumab Biosimilar) Products
- Target Type
- Biosimilar
- Background
- Tumor necrosis factor alpha
- UniProt
- P01375
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