Recombinant SIRPG antibody

Catalog No. ABIN7543055

Product Details anti-SIRPG Antibody

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This SIRPG antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Purpose: Anti-SIRP g [OX-119], Rabbit IgG, kappa

Specificity: Hybridoma supernatants were initially screened by ELISA, using SIRPg-CD4, human SIRPa-Fc, human SIRPb-Fc or CD4 to eliminate hybridomas with cross-reactivity to rat CD4, human SIRPa, and SIRPb. There is some marginal binding of OX-119 to SIRPb transfected 293T cells. SIRPg binds CD47. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells..

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: OX-119 was prepared by immunizing BALB/c mice with a chimeric protein made up of human SIRPg and a leader peptide of rat CD4 domain (SIRPg-CD4 ).

Clone: OX-119

Isotype: IgG kappa

Application Details

Application Notes: OX-119 is able to perform staining of peripheral blood myeloid cells (PBMCs) and block SIRPg-mediated apoptosis. OX-119 can also be used in IP, FC and ELISA.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for SIRPG

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Alternative Name: SIRP g (SIRPG Products)

Background: CD172g, OX119, MRC OX-119, MRC OX119, SIRP-g, SIRPg, SIRP gamma, SIRP-gamma, CD172 antigen-like family member B, signal-regulatory protein beta, CD172-g

UniProt: Q9P1W8