Recombinant SIRPG antibody
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- Target See all SIRPG Antibodies
- SIRPG (Signal-Regulatory Protein gamma (SIRPG))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SIRPG antibody is un-conjugated
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Application
- ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)
- Purpose
- Anti-SIRP g [OX-119], Mouse IgG1, kappa
- Specificity
- Hybridoma supernatants were initially screened by ELISA, using SIRPg-CD4, human SIRPa-Fc, human SIRPb-Fc or CD4 to eliminate hybridomas with cross-reactivity to rat CD4, human SIRPa, and SIRPb. There is some marginal binding of OX-119 to SIRPb transfected 293T cells. SIRPg binds CD47. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells..
- Characteristics
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
- Purification
- Protein A affinity purified
- Immunogen
- OX-119 was prepared by immunizing BALB/c mice with a chimeric protein made up of human SIRPg and a leader peptide of rat CD4 domain (SIRPg-CD4 ).
- Clone
- OX-119
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product SIRPG Primary Antibody
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- Application Notes
- OX-119 is able to perform staining of peripheral blood myeloid cells (PBMCs) and block SIRPg-mediated apoptosis. OX-119 can also be used in IP, FC and ELISA.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- SIRPG (Signal-Regulatory Protein gamma (SIRPG))
- Alternative Name
- SIRP g (SIRPG Products)
- Background
- CD172g, OX119, MRC OX-119, MRC OX119, SIRP-g, SIRPg, SIRP gamma, SIRP-gamma, CD172 antigen-like family member B, signal-regulatory protein beta, CD172-g
- UniProt
- Q9P1W8
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