INPPL1 antibody (AA 701-800)

Catalog No. ABIN722200

Product Details anti-INPPL1 Antibody

Target: INPPL1 (Inositol Polyphosphate Phosphatase-Like 1 (INPPL1))

Binding Specificity: AA 701-800

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INPPL1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human INPPL1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for INPPL1

Target: INPPL1 (Inositol Polyphosphate Phosphatase-Like 1 (INPPL1))

Alternative Name: INPPL1 (INPPL1 Products)

Synonyms: OPSMD antibody, SHIP2 antibody, 51C antibody, Ship2 antibody, inppl1 antibody, si:bz46j2.7 antibody, si:rp71-46j2.7 antibody, INPPL1 antibody, fc58a04 antibody, wu:fc58a04 antibody, zgc:198309 antibody, inositol polyphosphate phosphatase like 1 antibody, inositol polyphosphate phosphatase-like 1 antibody, inositol polyphosphate phosphatase-like 1b antibody, phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2B antibody, inositol polyphosphate phosphatase-like 1a antibody, INPPL1 antibody, Inppl1 antibody, inppl1b antibody, LOC100180571 antibody, inppl1a antibody

Background:

Synonyms: OPSMD, SHIP2, Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2, Inositol polyphosphate phosphatase-like protein 1, INPPL-1, Protein 51C, SH2 domain-containing inositol 5'-phosphatase 2, SH2 domain-containing inositol phosphatase 2, SHIP-2, INPPL1

Background: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity. May act by regulating AKT2, but not AKT1, phosphorylation at the plasma membrane. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submembraneous actin by hydrolyzing PtdIns(3,4,5)P3 thereby regulating membrane ruffling. Regulates cell adhesion and cell spreading. Required for HGF-mediated lamellipodium formation, cell scattering and spreading. Acts as a negative regulator of EPHA2 receptor endocytosis by inhibiting via PI3K-dependent Rac1 activation. Acts as a regulator of neuritogenesis by regulating PtdIns(3,4,5)P3 level and is required to form an initial protrusive pattern, and later, maintain proper neurite outgrowth. Acts as a negative regulator of the FC-gamma-RIIA receptor (FCGR2A). Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Involved in EGF signaling pathway. Upon stimulation by EGF, it is recruited by EGFR and dephosphorylates PtdIns(3,4,5)P3. Plays a negative role in regulating the PI3K-PKB pathway, possibly by inhibiting PKB activity.

Gene ID: 3636

UniProt: O15357

Pathways: Platelet-derived growth Factor Receptor Signaling