Nuclease antibody

Catalog No. ABIN7540666

Product Details anti-Nuclease Antibody

Target: Nuclease

Reactivity: Serratia marcescens

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nuclease antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: Nuclease/NucA Antibody

Cross-Reactivity (Details): This antibody will react with endonuclease from S.

Purification: This antibody was purified from monospecific antiserum by Protein A chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Nuclease/NucA was prepared by repeated immunizations with recombinant benzonase protein produced in E. coli.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:21,600 - 1:22,600

Western_Blot_Dilution: 1:1000

Comment:

Anti-Nuclease/NucA has been tested by ELISA and western blot. Expect a band ~ 28 kDa in size corresponding to endonuclease by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Nuclease

Target: Nuclease

Alternative Name: nucA (Nuclease Products)

Synonyms: F15D2.37 antibody, F15D2_37 antibody, 5'-3' exonuclease family protein antibody, Nuclease antibody, AT1G29630 antibody, Nuc antibody

Background: Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA,Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35 °C and 44 °C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.

UniProt: P13717