KDELR1 antibody (C-Term)

Catalog No. ABIN7540480

Supplier Product No.: 200-301-f44

Product Details anti-KDELR1 Antibody

Target: KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))

Binding Specificity: C-Term

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This KDELR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-f44

Supplier: Rockland

Purpose: HDEL Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with HDEL from Drosophilia, yeast, Saccharomyce, and Plants (Barnyard grass, beet, cotton, mung bean, sorghum, wheat) based on 100 % homology with the immunizing sequence.

Purification: Anti-HDEL Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: HDEL Antibody was produced in mice by repeated immunizations raised against a synthetic HDEL synthetic peptide corresponding to the C-terminus of yeast Bip.

Clone: 2E7

Isotype: IgG2b

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:50-1:500

Western_Blot_Dilution: 1:1000-1:2000

Comment:

Anti-HDEL Antibody is tested for use in WB and IF microscopy. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for KDELR1

Target: KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))

Alternative Name: ERD2 (KDELR1 Products)

Background: H-D-E-L, endoplasmic reticulum, luminal ER protein retention,HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat.

Pathways: Maintenance of Protein Location