DNAJB9 antibody

Catalog No. ABIN7539265

Product Details anti-DNAJB9 Antibody

Target: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB9 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Mdg-1 antibody

Purification: Protein-A purified

Immunogen: Recombinant human Mdg-1

Application Details

Application Notes: Western Blot: Use 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for DNAJB9

Target: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Alternative Name: Mdg-1 (DNAJB9 Products)

Synonyms: ERdj4 antibody, MDG-1 antibody, MDG1 antibody, MST049 antibody, MSTP049 antibody, AA408011 antibody, AA673251 antibody, AA673481 antibody, AW556981 antibody, Mdg1 antibody, mDj7 antibody, DnaJ heat shock protein family (Hsp40) member B9 antibody, DNAJB9 antibody, Dnajb9 antibody

Background: Microvascular endothelial differentiation gene 1 protein, DnaJ homolog subfamily B member 9, ERdj4,Angiogenesis research has focused on receptors and ligands mediating endothelial cell proliferation and migration. Little is known about the molecular mechanisms that are involved in converting endothelial cells from a proliferative to a differentiated state. Microvascular differentiation gene 1 (Mdg1) has been isolated from differentiating microvascular endothelial cells that had been cultured in collagen type I gels (3D culture). In adult human tissue Mdg1 is expressed in endothelial and epithelial cells. Sequence analysis of the full-length cDNA revealed that the N-terminal region of the putative Mdg1-protein exhibits a high sequence similarity to the J-domain of Hsp40 chaperones. It was shown that this region functions as a bona fide J-domain as it can replace the J-domain of Escherichia coli DnaJ-protein. Mdg1 is also upregulated in primary endothelial and mesangial cells when subjected to various stress stimuli. GFP-Mdg1 fusion constructs showed the Mdg1-protein to be localized within the cytoplasm under control conditions. Stress induces the translocation of Mdg1 into the nucleus, where it accumulates in nucleoli. Costaining with Hdj1, Hdj2, Hsp70, and Hsc70 revealed that Mdg1 colocalizes with Hsp70 and Hdj1 in control and stressed HeLa cells. These data suggest that Mdg1 is involved in the control of cell cycle arrest taking place during terminal cell differentiation and under stress conditions.

Gene ID: 4189

NCBI Accession: NM_012328, NP_036460

UniProt: Q9UBS3

Pathways: ER-Nucleus Signaling