AGGF1 antibody

Catalog No. ABIN7539232

Product Details anti-AGGF1 Antibody

Target: AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1 (AGGF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGGF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: AGGF1 antibody

Purification: Protein A purified

Immunogen: recombinant human AGGF1 (fragment)

Isotype: IgG

Application Details

Application Notes: WB: Use 1-5 μg/mL, IHC: 1:50

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for AGGF1

Target: AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1 (AGGF1))

Alternative Name: AGGF1 (AGGF1 Products)

Synonyms: RGD1310888 antibody, AGGF1 antibody, LOC100037685 antibody, im:7146636 antibody, zgc:152959 antibody, DKFZp459G1317 antibody, GPATC7 antibody, GPATCH7 antibody, HSU84971 antibody, HUS84971 antibody, VG5Q antibody, 2010009L17Rik antibody, 2310029P06Rik antibody, AW112072 antibody, Peg3 antibody, angiogenic factor with G patch and FHA domains 1 antibody, angiogenic factor with G-patch and FHA domains 1 antibody, Aggf1 antibody, AGGF1 antibody, aggf1 antibody, CC1G_06398 antibody

Background: Angiogenic factor VG5Q, hVG5Q, G patch domain-containing protein 7, Vasculogenesis gene on 5q protein,AGGF1, also known as VG5Q, was identified by its association with Klippel Trenaunay syndrome (KTS), a congenital vascular morphogenesis disorder (1 3). AGGF1 is expressed by vascular endothelial cells in many tissues (1). It appears to be secreted and promotes endothelial cell proliferation following interactions with endothelial cell surfaces (1). AGGF1 also directly interacts with TWEAK (1), a TNF superfamily ligand with angiogenic properties (8). It was shown that AGGF1 is involved in establishing venous identity in zebrafish embryos. Overexpression of AGGF1 led to increased angiogenesis and increased lumen diameter of veins, whereas knockdown of AGGF1 expression resulted in defective vasculogenesis and angiogenesis. Overexpression of AGGF1 increased expression of venous markers (e.g. VEGFR-3/FLT4), but had little effect on arterial markers (e.g. Notch5). Knockdown of AGGF1 expression resulted in a loss of venous identity (loss of expression of VEGFR-3/FLT4, Ephb4 and Dab2), but had no effect on the expression of arterial development. It was further shown that AGGF1 activates AKT, and that decreased AGGF1 expression inhibits AKT activation. Overexpression of constitutively active AKT rescues the loss of venous identity caused by AGGF1 downregulation. These results indicate that AGGF might be an angiogenic factor with an important role in the specification of vein identity and suggests that AGGF1-mediated AKT signaling is responsible for establishing venous cell fate.

Gene ID: 55109

UniProt: Q8N302