Dysferlin antibody (AA 1999-2016)

Catalog No. ABIN7538896

Product Details anti-Dysferlin Antibody

Target: Dysferlin (DYSF)

Binding Specificity: AA 1999-2016

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dysferlin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Lyophilized Mouse Monoclonal Antibody Dysferlin

Specificity: Reactive with the dysferlin molecule in human skeletal muscle. Also present in many non-muscle tissues.

Characteristics: This product is intended for the qualitative identification by light microscopy of Dysferlin by immunohistochemistry.

Purification: Tissue culture supernatant

Immunogen: Synthetic peptide containing amino acids 1999-2016 of the human dysferlin molecule.

Clone: Ham1-7B6

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections: Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. This is provided as a guide and users should determine their own optimal working dilutions. NCL-Hamlet requires the sections to be fixed in acetone/ methanol at a ratio of 1:1 for 4 minutes at 25°C prior to incubation with the primary antibody. Immunohistochemistry on paraffin sections. Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. High temperature antigen retrieval using 0.01 M citrate retrieval solution ( pH 6.0) is recommended. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.

Concentration: 180 mg/L

Buffer: This product is a lyophilized tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for Dysferlin

Target: Dysferlin (DYSF)

Alternative Name: Dysferlin (DYSF Products)

Synonyms: DYSF antibody, fb73b05 antibody, wu:fb73b05 antibody, si:rp71-50c18.1 antibody, DKFZp459E1226 antibody, 2310004N10Rik antibody, AI604795 antibody, D6Pas3 antibody, mFLJ00175 antibody, FER1L1 antibody, LGMD2B antibody, MMD1 antibody, dysferlin antibody, dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) antibody, myoferlin antibody, DYSF antibody, dysf antibody, LOC589501 antibody, Dysf antibody