Dysferlin antibody (AA 1999-2016)

Catalog No. ABIN7538896

Product Details anti-Dysferlin Antibody

Target: Dysferlin (DYSF)

Binding Specificity: AA 1999-2016

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dysferlin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Lyophilized Mouse Monoclonal Antibody Dysferlin

Specificity: Reactive with the dysferlin molecule in human skeletal muscle. Also present in many non-muscle tissues.

Characteristics: This product is intended for the qualitative identification by light microscopy of Dysferlin by immunohistochemistry.

Purification: Tissue culture supernatant

Immunogen: Synthetic peptide containing amino acids 1999-2016 of the human dysferlin molecule.

Clone: Ham1-7B6

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections: Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. This is provided as a guide and users should determine their own optimal working dilutions. NCL-Hamlet requires the sections to be fixed in acetone/ methanol at a ratio of 1:1 for 4 minutes at 25°C prior to incubation with the primary antibody. Immunohistochemistry on paraffin sections. Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. High temperature antigen retrieval using 0.01 M citrate retrieval solution ( pH 6.0) is recommended. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.

Concentration: 180 mg/L

Buffer: This product is a lyophilized tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for Dysferlin

Target: Dysferlin (DYSF)

Alternative Name: Dysferlin (DYSF Products)