Estrogen Receptor alpha antibody

Catalog No. ABIN7538886

Product Details anti-Estrogen Receptor alpha Antibody

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Purpose: Liquid Mouse Monoclonal Antibody Estrogen Receptor

Specificity: Human estrogen receptor.

Characteristics: This product is intended for the qualitative identification by light microscopy of Estrogen Receptor molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to the full length alpha form of the human estrogen receptor molecule.

Clone: 6F11

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:50 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 67.5 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor (ESR1 Products)

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly