alpha-Thrombin antibody

Catalog No. ABIN7536659

Product Details anti-alpha-Thrombin Antibody

Target: alpha-Thrombin

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This alpha-Thrombin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Neutralization (Neut), Immunodepletion (IDe), Immunoelectrophoresis (IEP)

Purpose: Thrombin Polyclonal Antibody

Specificity: Human Thrombin, alpha-thrombin

Purification: Fractionation of IgG from antiserum

Immunogen: Thrombin prepared from purified human prothrombin, active site blocked with PPACK.

Isotype: IgG

Application Details

Application Notes: Immunoblot 5-25 μg/mL, ELISA capture 20-50 μg/mL

Comment:

Whole IgG is a base level product and refers to IgG antibodies purified from antiserum using multiple precipitation and dialysis steps. The purity of the IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v). The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one millilitre of antiserum. This level of product is generally intended for use in applications such as immunoprecipitation, immunoelectrophoresis, immuno-depletion and activity neutralization assays.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Allow product to warm to room temperature and gently mix before use

Concentration: 10 mg/mL

Buffer: 10 mM HEPES, pH 7.4, 150 mM NaC, 50 % (v/v) glycerol

Storage: -20 °C

Storage Comment: Store between -10°C and -20°C

Target Details for alpha-Thrombin

Target: alpha-Thrombin

Alternative Name: Thrombin (alpha-Thrombin Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, coagulation factor II, thrombin antibody, F2 antibody