BIP/GRP78 antibody

Catalog No. ABIN7536640

Product Details anti-BIP/GRP78 Antibody

Target: BIP/GRP78 (Luminal-Binding Protein (BIP/GRP78))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This BIP/GRP78 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunodepletion (IDe), Immunoelectrophoresis (IEP), Neutralization (Neut)

Purpose: GRP78 Polyclonal Antibody

Specificity: Human GRP78 (HSPA5) (Heat Shock 70 kDa Protein 5 (Glucose-Regulated Protein, 78 kDa)

Purification: Antigen-specific affinity purification

Immunogen: Recombinant human GRP78

Isotype: IgG

Application Details

Application Notes: Immunoblot 0.5-5 μg/mL, ELISA capture 10 μg/mL

Comment:

Affinity-purified IgG are antibodies further purified using immobilized antigen. Affinity-purification is an enrichment process in which whole IgG is passed over a resin to which antigen has been covalently attached. Non-reactive antibodies are washed away and specific antibodies are eluted under conditions that disrupt the antigen-antibody interaction. The result is a very high-purity product with a substantially higher titre than whole IgG. This level of product is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Allow product to warm to room temperature and gently mix before use

Concentration: 1 mg/mL

Buffer: 10 mM HEPES, pH 7.4, 150 mM NaC, 50 % (v/v) glycerol

Storage: -20 °C

Storage Comment: Store between -10°C and -20°C

Target Details for BIP/GRP78

Target: BIP/GRP78 (Luminal-Binding Protein (BIP/GRP78))

Alternative Name: Glucose-Regulated Protein 78 (GRP78) (BIP/GRP78 Products)