RNF43 antibody (AA 41-140)

Catalog No. ABIN721600

Product Details anti-RNF43 Antibody

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Binding Specificity: AA 41-140

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF43 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RNF43

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RNF43

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Alternative Name: RNF43 (RNF43 Products)

Synonyms: RNF124 antibody, URCC antibody, 4732452J19Rik antibody, RGD1305204 antibody, ring finger protein 43 antibody, RNF43 antibody, Rnf43 antibody

Background:

Synonyms: URCC, RNF124, E3 ubiquitin-protein ligase RNF43, RING finger protein 43, RNF43

Background: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.

Gene ID: 54894

UniProt: Q68DV7

Pathways: WNT Signaling