MHC Class II antibody (Biotin)
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- Target See all MHC Class II (MHC2) Antibodies
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This MHC Class II antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Anti-Ms MHC Class II Biotin
- Specificity
- The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
- Purification
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
- Immunogen
- Activated C57BL/6 mouse spleen cells
- Clone
- M5-114
- Isotype
- IgG2b
- Top Product
- Discover our top product MHC2 Primary Antibody
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anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody
MHC2 Reactivity: Mouse FACS, WB, IHC (fro), IP, IHC (p), Func Host: Rat Monoclonal M5-114 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 Reactivity: Mouse FACS Host: Rat Monoclonal M5-114 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 Reactivity: Chicken FACS Host: Mouse Monoclonal 2G11 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibodyMHC2 Reactivity: Mouse, Human WB, IHC (fro), IHC (p), IF (cc), IF (p), ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 Reactivity: Chicken FACS Host: Mouse Monoclonal 2G11 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 Reactivity: Mouse FACS Host: Rat Monoclonal NIMR-4 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)MHC2 Reactivity: Mouse FACS Host: Rat Monoclonal NIMR-4 APC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (SPRD)MHC2 Reactivity: Mouse FACS Host: Rat Monoclonal NIMR-4 SPRD
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)MHC2 Reactivity: Chicken FACS Host: Mouse Monoclonal 2G11 Biotin
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 Reactivity: Chicken FACS Host: Mouse Monoclonal 2G11 FITC
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- Application Notes
- Flow cytometry: Recommended dilution: 1-4 μg/mL.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
- Alternative Name
- MHC Class II (MHC2 Products)
- Background
- MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
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