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mCherry antibody

Name: mCherry antibody
Brand: antibodies-online
SKU: ABIN7505996
Price: 417.45 USD
Availability: InStock

Reactivity: Anaplasma marginale WB, ICC, FACS Host: Rabbit Polyclonal unconjugated

Western blotting analysis of mCherry fluorescent protein using rabbit polyclonal PAb (918) on lysates of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI, negative control) under reducing and non-reducing conditions. (mCherry antibody)

Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained using anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC). (mCherry antibody)

FACS

Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension. (mCherry antibody)

FACS

3 images

Catalog No. ABIN7505996

Datasheet as PDF

Target See all mCherry products

mCherry (mCherry Fluorescent Protein)
Reactivity
21

14

3

2
Anaplasma marginale
Host
20

14

2

1
Rabbit
Compatible Secondaries
Clonality
24

12
Polyclonal
Conjugate
19

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This mCherry antibody is un-conjugated
Application
34

9

5

4

3

3

1

1

1
Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose

Anti-mCherry purified

Specificity

The rabbit polyclonal antibody PAb (918) recognizes a red fluorescent protein tag mCherry.

Purification

Purified by ligand affinity chromatography.

Immunogen

mCherry protein from Anaplasma marginale
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Reactivity: Discosoma WB Host: Rabbit Polyclonal unconjugated

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Reactivity: Discosoma WB Host: Mouse Polyclonal unconjugated

anti-mCherry Fluorescent Protein antibody
Reactivity: Discosoma WB Host: Mouse Monoclonal unconjugated

anti-mCherry Fluorescent Protein antibody
Reactivity: Discosoma WB, IF, ICC Host: Rabbit Polyclonal unconjugated

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Reactivity: Discosoma WB, IF, ICC Host: Chicken Polyclonal unconjugated

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Reactivity: Discosoma WB, IP Host: Mouse Monoclonal unconjugated

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Reactivity: Discosoma WB, IF, ICC, IHC Host: Mouse Monoclonal 1C51 unconjugated

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Reactivity: Various Species WB Host: Rabbit Polyclonal unconjugated

anti-mCherry Fluorescent Protein antibody
Reactivity: Various Species WB, IF Host: Mouse Monoclonal 4-00E-003 unconjugated

Application Notes

Flow cytometry: Recommended dilution: 1-4 μg/mL, extracellular staining or intracellular staining - depending on expression.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Target

mCherry (mCherry Fluorescent Protein)

Alternative Name

mCherry (mCherry Products)

Background

The mCherry is a red fluorescent protein with excitation maximum 587 nm and emission maximum 610 nm. It has around 28 kDa, and it is being used as a fluorescent tag in expression systems.

UniProt

X5DSL3