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IL-2 antibody (APC)

Name: IL-2 antibody (APC)
Brand: antibodies-online
SKU: ABIN7505990
Price: 462.23 USD
Availability: InStock

IL2 Reactivity: Human FACS Host: Mouse Monoclonal 35C3 APC

Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (IL-2 antibody (APC))

FACS

Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue antibody (4 μL reagent / 100 μL of peripheral whole blood) and intracellular staining using anti-human IL-2 (35C3) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (IL-2 antibody (APC))

FACS

Separation of human IL-2 positive CD3 positive lymphocytes (red-filled) from IL-2 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (IL-2 antibody (APC))

FACS

3 images

Catalog No. ABIN7505990

Datasheet as PDF

Target See all IL-2 (IL2) Antibodies

IL-2 (IL2) (Interleukin 2 (IL2))
Reactivity
133

86

43

14

9

8

6

6

5

4

4

4

3

2

1

1

1

1
Human
Host
117

78

57

11

4

2

1
Mouse
Compatible Secondaries
Clonality
137

131

1
Monoclonal
Conjugate
144

36

23

20

15

10

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This IL-2 antibody is conjugated to APC
Application
159

127

64

60

39

27

27

21

14

14

13

13

13

11

10

8

6

6

5

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS)

Purpose

Anti-Hu IL-2 APC

Specificity

The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2, secreted or intracellular).

Purification

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

recombinant human IL-2

Clone

35C3

Isotype

IgG2b kappa

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Application Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions

For Research Use only
Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Target

IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name

IL-2 (IL2 Products)

Synonyms

IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody

Background

Interleukin 2,IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.,Interleukin 2

Gene ID

3558

UniProt

P60568

Pathways

JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation