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- Target See all IgE Antibodies
- IgE
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This IgE antibody is conjugated to APC
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Application
- Flow Cytometry (FACS)
- Purpose
- Anti-Hu IgE APC
- Specificity
- The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
- Purification
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- Purified human IgE.
- Clone
- 4H10
- Isotype
- IgG1
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anti-IgE antibody (PE)
Reactivity: Human FACS Host: Mouse Monoclonal 4H10 PE
anti-IgE antibody (Biotin)Reactivity: Human ELISA, FACS Host: Mouse Monoclonal BE5 Biotin
anti-IgE (AA 1-421) antibodyReactivity: Mouse WB, ICC, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-IgE (AA 246-275) antibodyReactivity: Human WB Host: Rabbit Polyclonal RB32377 unconjugated
anti-IgE antibodyReactivity: Human ELISA, ICC, IHC, FACS Host: Rabbit Monoclonal RM122 unconjugated Recombinant Antibody
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- Application Notes
- Flow cytometry: Recommended dilution: 0.5-3 μg/mL
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- IgE
- Abstract
- IgE Products
- Synonyms
- IGH2 antibody, IgE antibody, Gm900 antibody, immunoglobulin heavy chain (epsilon polypeptide) antibody, immunoglobulin heavy constant epsilon antibody, Immunoglobulin heavy constant epsilon antibody, Ighe antibody, IGHE antibody
- Background
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
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