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Fc gamma RII (CD32) antibody (FITC)

CD32 Reactivity: Human FACS Host: Mouse Monoclonal 3D3 FITC
Catalog No. ABIN7505913
  • Target See all Fc gamma RII (CD32) Antibodies
    Fc gamma RII (CD32)
    Reactivity
    • 120
    • 9
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    • 5
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    • 3
    Human
    Host
    • 69
    • 60
    Mouse
    Clonality
    • 66
    • 60
    • 3
    Monoclonal
    Conjugate
    • 44
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    • 5
    • 3
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    • 3
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    • 2
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    FITC
    Application
    • 69
    • 54
    • 23
    • 20
    • 12
    • 11
    • 8
    • 7
    • 6
    • 3
    • 2
    • 2
    • 1
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    • 1
    Flow Cytometry (FACS)
    Purpose
    Anti-Hu CD32 FITC
    Specificity
    The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    purified glycosylated recombinant human FcgammaRIIa2
    Clone
    3D3
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD32 Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
    Restrictions
    For Research Use only
  • Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Target
    Fc gamma RII (CD32)
    Alternative Name
    CD32 (CD32 Products)
    Synonyms
    CD32 antibody, CD32B antibody, FCG2 antibody, FCGR2 antibody, IGFR2 antibody, FCGR2B antibody, AI528646 antibody, F630109E10Rik antibody, Fc[g]RII antibody, FcgRII antibody, Fcgr2 antibody, Fcgr2a antibody, Fcr-2 antibody, Fcr-3 antibody, Ly-17 antibody, Ly-m20 antibody, LyM-1 antibody, Lym-1 antibody, FCRII antibody, FcRII antibody, Fc fragment of IgG receptor IIb antibody, Fc receptor, IgG, low affinity IIb antibody, FCGR2B antibody, Fcgr2b antibody
    Background
    Fc fragment of IgG receptor IIa,CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).,FCG2, FCGR2A, IGFR2
    Gene ID
    2212
    UniProt
    P12318
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