This Influenza A Virus Matrix Protein 1 antibody is conjugated to Peroxidase (POD)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
Specificity
Purified matrix protein (M1)
Cross-Reactivity (Details)
Does not react with HEp-2 cells by indirect immunofluorescence. Does not react with the M2 matrix protein
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
M1
Reactivity: Influenza A Virus
ELISA
Host: Mouse
Monoclonal
unconjugated
Application Notes
TITER : >1:160 by indirect immunofluorescence, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.