This Helicobacter Pylori antibody is conjugated to FITC
Application
Functional Studies (Func)
Specificity
Whole cell lysate
Cross-Reactivity (Details)
This antiserum has not been absorbed and may react with related microorganisms.
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
H. pylori
Reactivity: Helicobacter pylori
ELISA, WB, IHC, FM
Host: Rabbit
Polyclonal
Biotin
Application Notes
TITER : >1:1,000 in IFA, >1:5,000 in ELISA, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.