This Candida albicans antibody is conjugated to Biotin
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
Specificity
Recognizes numerous proteins in a soluble C. albicans extract (IEP).
Cross-Reactivity (Details)
This antiserum has not been absorbed and does crossreact with other yeasts. UNINFECTED CELL REACTIVITY: Negative vs. human serum, urine & spinal fluid
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
TITER : > 1: in indirect IFA. Use neat in gel-precipitin reactions. Applications in double-diffusion, CIE, IFA, ELISA & IHC, Possible applications for this product include avidin and streptavidin amplification systems for immunohistochemistry, ELISA, fluorescence microscopy and immunoblotting. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1 % sodium azide preservative. No stabilizing proteins have been added.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.