This Legionella Pneumophila antibody is conjugated to FITC
Application
Functional Studies (Func)
Specificity
All antigens of intact microorganism
Cross-Reactivity (Details)
Antiserum is not absorbed and may crossreact with related microorganisms. Reactive with L. pneumophila serogroups 1-12 in IFA
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
Immunogen
A whole cell preparation of Legionella pneumophila
Reactivity: Legionella pneumophila
ELISA, IFA
Host: Rabbit
Polyclonal
HRP
Application Notes
TITER : > 1:800 by indirect IFA vs. acetone-fixed organsims, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.