ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
Specificity
Gp120 (predominate gp120 band in WB)
Cross-Reactivity (Details)
Antiserum does not cross-react with human T or B cells UNINFECTED CELL REACTIVITY: Antiserum is not reactive with human or bovine sera
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
HIV-1 gp120
Reactivity: Human Immunodeficiency Virus (HIV)
WB, IHC, EIA
Host: Goat
Polyclonal
Peroxidase (POD)
Application Notes
TITER : > 1:1,000 by western blot immunoassay, Possible applications for this product include avidin and streptavidin amplification systems for immunohistochemistry, ELISA, fluorescence microscopy and immunoblotting. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1 % sodium azide preservative. No stabilizing proteins have been added.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.