Elementary Bodies antibody (Peroxidase (POD))

Catalog No. ABIN7478221

Product Details anti-Elementary Bodies Antibody (Peroxidase (POD))

Target: Elementary Bodies (EB) (Chlamydia trachomatis Elementary Bodies (EB))

Reactivity: Chlamydia trachomatis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elementary Bodies antibody is conjugated to Peroxidase (POD)

Application: Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Purified elementary bodies, disrupted

Cross-Reactivity (Details): Cross-reacts with Chlamydia psittacii & Chlamydia pneumoniae (TWAR) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2 cells and egg yolk sac

Purification: This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: L2 + other serovar groups

Isotype: IgG

Application Details

Application Notes: TITER : 1:1,000-3,000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3), This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details for Elementary Bodies

Target: Elementary Bodies (EB) (Chlamydia trachomatis Elementary Bodies (EB))

Alternative Name: Chlamydia trachomatis Elementary Bodies (EB Products)