Human Adenovirus Hexon (HAdV Hexon) antibody (Peroxidase (POD))
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- Target
- Human Adenovirus Hexon (HAdV Hexon)
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Reactivity
- Adenovirus
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Peroxidase (POD)
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Application
- Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- Numerous Adeno proteins including hexon
- Cross-Reactivity (Details)
- Reactive with Adeno types 1, 2, 3, 5, 6, 7a, 8, 31, 40 & 41. Others not tested. Does not crossreact with Para 1-3, Infl. A & B, RSV, hMPV & Rhinovirus (type 16) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2, LLCMK2 & Vero cells by indirect immunofluorescence
- Purification
- This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
- Immunogen
- gradient purified, disrupted Adenovirus type 5 virions
- Isotype
- IgG
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- Application Notes
- TITER : > 1: 5,000 by ELISA, > 1:8,000 in IFA, > 1:1,000 in western blot immunoassay, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- Target
- Human Adenovirus Hexon (HAdV Hexon)
- Alternative Name
- Human Adenovirus Hexon
- Target Type
- Viral Protein
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