Human Adenovirus Hexon (HAdV Hexon) antibody (FITC)
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- Target
- Human Adenovirus Hexon (HAdV Hexon)
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Reactivity
- Adenovirus
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- Functional Studies (Func)
- Specificity
- Numerous Adeno proteins including hexon
- Cross-Reactivity (Details)
- Reactive with Adeno types 1, 2, 3, 5, 6, 7a, 8, 31, 40 & 41. Others not tested. Does not crossreact with Para 1-3, Infl. A & B, RSV, hMPV & Rhinovirus (type 16) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2, LLCMK2 & Vero cells by indirect immunofluorescence
- Purification
- This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
- Immunogen
- gradient purified, disrupted Adenovirus type 5 virions
- Isotype
- IgG
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- Application Notes
- TITER : > 1: 5,000 by ELISA, > 1:8,000 in IFA, > 1:1,000 in western blot immunoassay, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.
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- Target
- Human Adenovirus Hexon (HAdV Hexon)
- Alternative Name
- Human Adenovirus Hexon
- Target Type
- Viral Protein
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