E. Coli antibody (Biotin)

Catalog No. ABIN7478179

Product Details anti-E. Coli Antibody (Biotin)

Target: E. Coli

Reactivity: E. coli

Host: Goat

Clonality: Polyclonal

Conjugate: This E. Coli antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Specificity: Many "O" and "K" antigenic serotypes of Escherichia coli

Cross-Reactivity (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Purification: This product consists of purified IgG fraction of neat antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Immunogen: Mixture of E. coli serotypes

Isotype: IgG

Application Details

Application Notes: TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Possible applications for this product include avidin and streptavidin amplification systems for immunohistochemistry, ELISA, fluorescence microscopy and immunoblotting. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1 % sodium azide preservative. No stabilizing proteins have been added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details for E. Coli

Target: E. Coli

Abstract: E. Coli Products

Target Type: Bacteria