E. Coli antibody (Peroxidase (POD))

Catalog No. ABIN7478178

Product Details anti-E. Coli Antibody (Peroxidase (POD))

Target: E. Coli

Reactivity: E. coli

Host: Goat

Clonality: Polyclonal

Conjugate: This E. Coli antibody is conjugated to Peroxidase (POD)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Many "O" and "K" antigenic serotypes of Escherichia coli

Cross-Reactivity (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Purification: This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: Mixture of E. coli serotypes

Isotype: IgG

Application Details

Application Notes: TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details for E. Coli

Target: E. Coli

Abstract: E. Coli Products

Target Type: Bacteria