E. Coli antibody (FITC)
Reactivity: E. coli
Func
Host: Goat
Polyclonal
FITC
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- Target See all E. Coli products
- E. Coli
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Reactivity
- E. coli
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This E. Coli antibody is conjugated to FITC
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Application
- Functional Studies (Func)
- Specificity
- Many "O" and "K" antigenic serotypes of Escherichia coli
- Cross-Reactivity (Details)
- Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
- Purification
- This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
- Immunogen
- Mixture of E. coli serotypes
- Isotype
- IgG
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- Application Notes
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TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ). - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.
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- Target
- E. Coli
- Abstract
- E. Coli Products
- Target Type
- Bacteria
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