E. Coli antibody

Catalog No. ABIN7478172

Product Details anti-E. Coli Antibody

Target: E. Coli

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E. Coli antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Specificity: All "O" and "K" antigenic serotypes of Escherichia coli

Cross-Reactivity (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Purification: This product consists of the purified IgG fraction of the above antiserum.

Purity: 95 % by SDS-PAGE

Immunogen: Mixtures of all antigenic serotypes

Isotype: IgG

Application Details

Application Notes: TITER : > 1:2,000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Potential applications for this product are numerous including ELISA, fluorescence microscopy, immunoblotting and immunohistochemistry. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique. It is also suitable for conjugation purposes. Will remove E. coli proteins from recombinant preparations

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1 % sodium azide preservative. No stabilizing proteins have been added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details for E. Coli

Target: E. Coli

Abstract: E. Coli Products

Target Type: Bacteria