This Leptospira Biflexa antibody is conjugated to Peroxidase (POD)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
Specificity
All antigens
Purification
This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
Reactivity: Leptospira biflexa
IF
Host: Rabbit
Polyclonal
FITC
Application Notes
TITER : >1:500 in indirect FA This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.