Borrelia Burgdorferi antibody (Peroxidase (POD))

Catalog No. ABIN7478142

Product Details anti-Borrelia Burgdorferi Antibody (Peroxidase (POD))

Target: Borrelia Burgdorferi

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Borrelia Burgdorferi antibody is conjugated to Peroxidase (POD)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Specificity: Reactive Western Blot bands at 83kD, 41kD, 34kD and 31kD + additional low MW bands

Cross-Reactivity (Details): Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii

Purification: This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: Whole cell preparation from B. burgdorferi

Isotype: IgG

Application Details

Application Notes: TITER : >1:1,000 in Western Blot. >1:40,000 in avidin-biotin IFA
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays, Functions well in formalin fixed/paraffin embedded settings

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details for Borrelia Burgdorferi

Target: Borrelia Burgdorferi

Abstract: Borrelia Burgdorferi Products