Recombinant VWF antibody (AA 1815-1939)

Catalog No. ABIN7477621

Product Details anti-VWF Antibody

Target: VWF (Von Willebrand Factor (VWF))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 1815-1939

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This VWF antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)

Clone: MSVA-521R

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100

Comment:

Positive Control: Colon: A moderate to strong endothelial vWF immunostaining should be seen in a fraction of blood vessels.

Negative Control: Colon: vWF immunostaining should be completely absent in epithelial and muscular cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: von Willebrand Factor / vWF (VWF Products)

Background: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2,von Willebrand Disease (vWD),von Willebrand Factor / vWF antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P04275