Recombinant Villin 1 antibody (AA 600-700)
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- Target See all Villin 1 (VIL1) Antibodies
- Villin 1 (VIL1)
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 600-700
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Villin 1 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)
- Clone
- MSVA-459R
- Isotype
- IgG
- Top Product
- Discover our top product VIL1 Primary Antibody
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.
Negative Control: Colon: Villin staining should be absent in all non-epithelial cells.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- Villin 1 (VIL1)
- Alternative Name
- Villin (VIL1 Products)
- Synonyms
- D2S1471 antibody, VIL antibody, Vil antibody, vil1 antibody, MGC64339 antibody, VIL1 antibody, vil1l antibody, zgc:55779 antibody, villin 1 antibody, villin 1 S homeolog antibody, VIL1 antibody, Vil1 antibody, vil1.S antibody, vil1 antibody
- Background
- VIL1, Villin-1, Villin1,Villin antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P09327
- Pathways
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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