Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Recombinant Villin 1 antibody (AA 600-700)

VIL1 Reactivity: Human IHC Host: Rabbit Monoclonal MSVA-459R unconjugated Recombinant Antibody
Catalog No. ABIN7477617
  • Target See all Villin 1 (VIL1) Antibodies
    Villin 1 (VIL1)
    Antibody Type
    Recombinant Antibody
    Binding Specificity
    • 16
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 600-700
    Reactivity
    • 73
    • 25
    • 19
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 46
    • 27
    Rabbit
    Clonality
    • 38
    • 35
    Monoclonal
    Conjugate
    • 45
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Villin 1 antibody is un-conjugated
    Application
    • 43
    • 26
    • 17
    • 16
    • 12
    • 8
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogen
    Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)
    Clone
    MSVA-459R
    Isotype
    IgG
    Top Product
    Discover our top product VIL1 Primary Antibody
  • Application Notes
    IHC 1:100-1:200
    Comment

    Positive Control: Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.

    Negative Control: Colon: Villin staining should be absent in all non-epithelial cells.

    Protocol
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target
    Villin 1 (VIL1)
    Alternative Name
    Villin (VIL1 Products)
    Synonyms
    D2S1471 antibody, VIL antibody, Vil antibody, vil1 antibody, MGC64339 antibody, VIL1 antibody, vil1l antibody, zgc:55779 antibody, villin 1 antibody, villin 1 S homeolog antibody, VIL1 antibody, Vil1 antibody, vil1.S antibody, vil1 antibody
    Background
    VIL1, Villin-1, Villin1,Villin antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    P09327
    Pathways
    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
You are here:
Support