Uroplakin 3B antibody

Catalog No. ABIN7477615

Product Details anti-Uroplakin 3B Antibody

Target: Uroplakin 3B (UPK3B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Uroplakin 3B antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-736M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Urinary bladder: A moderate to strong membranous Upk3b immunostaining should be seen in umbrella cells of the normal urothelium (the staining is usually limited to apical surface membrane of umbrella cells).

Negative Control: Colon: Upk3b immunostaining should be absent in all cells of the colon mucosa.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Uroplakin 3B

Target: Uroplakin 3B (UPK3B)

Alternative Name: Uroplakin 3B (UPK3B Products)

Background: FLJ32198, MGC10902, p35, UPIIIb,Uroplakin 3B antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q9BT76