TFF1 antibody (AA 1-88)

Catalog No. ABIN7477597

Product Details anti-TFF1 Antibody

Target: TFF1 (Trefoil Factor 1 (TFF1))

Binding Specificity: AA 1-88

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant fragment (around aa1-88) of human TFF1protein (exact sequence is proprietary)

Clone: MSVA-482M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Stomach: A strong cytoplasmic TFF1 staining should be seen in the gastric surface epithelium.

Negative Control: Kidney: TFF1 immunostaining should be absent in all cell types.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: TFF1 (TFF1 Products)

Background: BCEI, Breast Cancer Estrogen Inducible Protein, Gastrointestinal Trefoil Protein, Gastrointestinal trefoil protein pS2, HP1A, HPS2, pNR2, TFF1, Trefoil Factor 1,TFF1 / pS2 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P04155

Pathways: EGFR Signaling Pathway