Recombinant SOX9 antibody (AA 393-508)

Catalog No. ABIN7477587

Product Details anti-SOX9 Antibody

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 393-508

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary)

Clone: MSVA-709R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.

Negative Control: Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres - if present).

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9 (SOX9 Products)

Background: Campomelic Dysplasia Autosomal Sex Reversal (CMD1), SRA1, SRXX2, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX9,SOX9 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development