S100A12 antibody

Catalog No. ABIN7477583

Product Details anti-S100A12 Antibody

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A12 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-812M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Spleen: A significant number of inflammatory cells should show a strong S100A12 immunostaining while the majority of cells remain completely negative.

Negative Control: Colon: All epithelial and muscular cells must not show any S100A12 immunostaining. Few scattered S100A12 positive inflammatory cells can occur (mostly within blood vessels)

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for S100A12

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Alternative Name: S100A12 (S100A12 Products)

Background: CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6,S100A12 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P80511

Pathways: Toll-Like Receptors Cascades, S100 Proteins