Recombinant Melanoma gp100 antibody

Catalog No. ABIN7477565

Product Details anti-Melanoma gp100 Antibody

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-617R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Skin: A moderate to strong cytoplasmic PMEL immunostaining should be seen in all melanocytes of the skin.

Negative Control: Colon: PMEL immunostaining should be completely absent in all cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: PMEL (PMEL Products)

Synonyms: D12S53E antibody, ME20 antibody, ME20-M antibody, ME20M antibody, P1 antibody, P100 antibody, PMEL17 antibody, SI antibody, SIL antibody, SILV antibody, gp100 antibody, D10H12S53E antibody, D12S53Eh antibody, Pmel17 antibody, Si antibody, Silv antibody, gp87 antibody, RPE1 antibody, MMP115 antibody, silverb antibody, silver antibody, cb397 antibody, fdv antibody, pmel17 antibody, sb:cb397 antibody, silva antibody, wu:fc11g11 antibody, wu:fj24g11 antibody, zgc:136622 antibody, premelanosome protein antibody, premelanosome protein b antibody, premelanosome protein a antibody, PMEL antibody, Pmel antibody, pmelb antibody, pmela antibody

Background: 95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog,PMEL / gp100 / Melanosome antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P40967