Occludin antibody (AA 282-415)
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- Target See all Occludin (OCLN) Antibodies
- Occludin (OCLN)
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Binding Specificity
- AA 282-415
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Occludin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
- Clone
- MSVA-415M
- Isotype
- IgG
- Top Product
- Discover our top product OCLN Primary Antibody
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anti-Occludin (OCLN) (AA 431-522) antibody
OCLN Reactivity: Human, Mouse, Pig WB, ELISA, FACS, IF (cc), IF (p), IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Occludin (OCLN) (AA 263-522) antibodyOCLN Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Occludin (OCLN) (C-Term) antibodyOCLN Reactivity: Human WB, IHC (p), IHC (fro) Host: Goat Polyclonal unconjugated
anti-Occludin (OCLN) (AA 423-522) antibodyOCLN Reactivity: Human WB, ELISA, IP, IF Host: Mouse Monoclonal 1G7 unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN Reactivity: Human WB, IHC (f) Host: Mouse Monoclonal OCLN-2181 unconjugated
anti-Occludin (OCLN) (AA 1-522) antibodyOCLN Reactivity: Human WB, PLA Host: Rabbit Polyclonal unconjugated
anti-Occludin (OCLN) (AA 1-522) antibodyOCLN Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-Occludin (OCLN) (AA 285-430) antibodyOCLN Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Occludin (OCLN) (C-Term) antibodyOCLN Reactivity: Human, Mouse, Rat, Dog WB, ELISA, IHC, IF, IHC (p) Host: Mouse Monoclonal OC-3F10 unconjugated
anti-Occludin (OCLN) (AA 350-450) antibodyOCLN Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.
Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- Occludin (OCLN)
- Alternative Name
- Occludin (OCLN Products)
- Synonyms
- AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody
- Background
- BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- Q16625
- Pathways
- Cell-Cell Junction Organization, Hepatitis C
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