Recombinant Nucleolin antibody
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- Target See all Nucleolin (NCL) Antibodies
- Nucleolin (NCL)
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Nucleolin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant full-length human NCL protein
- Clone
- MSVA-623R
- Isotype
- IgG
- Top Product
- Discover our top product NCL Primary Antibody
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.
Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- Nucleolin (NCL)
- Alternative Name
- Nucleolin (NCL Products)
- Synonyms
- C23 antibody, B530004O11Rik antibody, D0Nds28 antibody, D1Nds28 antibody, Nucl antibody, wu:fa12d03 antibody, zgc:152810 antibody, nucl antibody, LOC100282923 antibody, LOC100283058 antibody, nucleolin antibody, nucleolin S homeolog antibody, NCL antibody, Ncl antibody, ncl.S antibody, ncl antibody, LOC100195537 antibody, LOC100282923 antibody, LOC100283058 antibody
- Background
- NCL, Nucl, Nucleolin, Protein C23,Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P19338
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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