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Recombinant Nucleolin antibody

NCL Reactivity: Human IHC Host: Rabbit Monoclonal MSVA-623R unconjugated Recombinant Antibody
Catalog No. ABIN7477541
  • Target See all Nucleolin (NCL) Antibodies
    Nucleolin (NCL)
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 128
    • 31
    • 30
    • 10
    • 9
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    • 8
    • 8
    • 6
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    • 1
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    Human
    Host
    • 87
    • 44
    • 1
    Rabbit
    Clonality
    • 88
    • 44
    Monoclonal
    Conjugate
    • 63
    • 12
    • 8
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    • 2
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    This Nucleolin antibody is un-conjugated
    Application
    • 104
    • 62
    • 58
    • 43
    • 43
    • 19
    • 17
    • 14
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    • 13
    • 10
    • 3
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    • 2
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogen
    Recombinant full-length human NCL protein
    Clone
    MSVA-623R
    Isotype
    IgG
    Top Product
    Discover our top product NCL Primary Antibody
  • Application Notes
    IHC 1:100-1:200
    Comment

    Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.

    Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.

    Protocol
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target
    Nucleolin (NCL)
    Alternative Name
    Nucleolin (NCL Products)
    Synonyms
    C23 antibody, B530004O11Rik antibody, D0Nds28 antibody, D1Nds28 antibody, Nucl antibody, wu:fa12d03 antibody, zgc:152810 antibody, nucl antibody, LOC100282923 antibody, LOC100283058 antibody, nucleolin antibody, nucleolin S homeolog antibody, NCL antibody, Ncl antibody, ncl.S antibody, ncl antibody, LOC100195537 antibody, LOC100282923 antibody, LOC100283058 antibody
    Background
    NCL, Nucl, Nucleolin, Protein C23,Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    P19338
    Pathways
    Ribonucleoprotein Complex Subunit Organization
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