ENO2/NSE antibody (AA 416-433)

Catalog No. ABIN7477539

Product Details anti-ENO2/NSE Antibody

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Binding Specificity: AA 416-433

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)

Clone: MSVA-451M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative.

Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: NSE gamma (ENO2 Products)

Background: ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE , -phospho-D-glycerate hydrolyase,NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P09104