Recombinant MSH2 antibody

Catalog No. ABIN7477531

Product Details anti-MSH2 Antibody

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant full-length human MSH2 protein

Clone: MSVA-902M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.

Negative Control: Colon adenocarcinoma with previously documented loss of MSH2 expression: no nuclear staining should be seen in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2 (MSH2 Products)

Synonyms: COCA1 antibody, FCC1 antibody, HNPCC antibody, HNPCC1 antibody, LCFS2 antibody, AI788990 antibody, wu:fc06b02 antibody, wu:fc13e09 antibody, zgc:55333 antibody, ATMSH2 antibody, MUTS homolog 2 antibody, msh2 antibody, mutS homolog 2 antibody, mutS homolog 2 (E. coli) antibody, MUTS homolog 2 antibody, mutS homolog 2 L homeolog antibody, MutS protein homolog 2 antibody, MSH2 antibody, Msh2 antibody, msh2 antibody, msh2.L antibody

Background: BAT26, COCA1, DNA mismatch repair protein Msh2, FCC1, hMSH2, HNPCC1, LCFS2, MSH2, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2,MSH2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response