Myeloperoxidase antibody

Catalog No. ABIN7477523

Product Details anti-Myeloperoxidase Antibody

Target: Myeloperoxidase (MPO)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant fragment of human MPO protein (exact sequence is proprietary)

Clone: MSVA-692M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Spleen: Numerous granulocytes with strong MPO staining should be seen within the red pulp of the spleen.

Negative Control: Colon: MPO immunostaining should be absent in all epithelial cells of the colon while MPO positive granulocytes may be seen within capillaries or in the stroma of the lamina propria.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: Myeloperoxidase / MPO (MPO Products)

Synonyms: mKIAA4033 antibody, MPO antibody, LOC100335032 antibody, POX2' antibody, XPOX2' antibody, mpo antibody, mpo-A antibody, pmr-1 antibody, pmr1 antibody, pox2 antibody, xpox2 antibody, myeloperoxidase antibody, myeloperoxidase L homeolog antibody, MPO antibody, Mpo antibody, LOC100335032 antibody, mpo.L antibody

Background: 4 kDa / 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain,Myeloperoxidase / MPO antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P05164

Pathways: Chromatin Binding