Recombinant MCM7 antibody (AA 195-319)

Catalog No. ABIN7477515

Product Details anti-MCM7 Antibody

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 195-319

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MCM7 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant human MCM7 protein fragment (aa195-319)

Clone: MSVA-507R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong nuclear MCM7 immunostaining should be seen in virtually all crypt base cells.

Negative Control: Colon: MCM7 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7 (MCM7 Products)

Background: CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Minichromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146,MCM7 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA