Recombinant MBP antibody (AA 150-250)

Catalog No. ABIN7477511

Product Details anti-MBP Antibody

Target: MBP (Myelin Basic Protein (MBP))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 150-250

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)

Clone: MSVA-390R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Brain: A fibrillar staining pattern should be seen.

Negative Control: Colon: MBP staining should be completely absent.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein (MBP Products)

Background: GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP,Myelin Basic Protein antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P02686