Recombinant Mammaglobin antibody
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- Target See all Mammaglobin products
- Mammaglobin
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Mammaglobin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant full-length human Mammaglobin (SCGB2A2) protein
- Clone
- MSVA-457R
- Isotype
- IgG
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Cervix uteri: a moderate to strong cytoplasmic staining of the endocervical glands should be seen while the squamous epithelium of the ektocervix remains negative.
Negative Control: Colon: All cells should not show any staining.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- Mammaglobin
- Abstract
- Mammaglobin Products
- Background
- Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin,Mammaglobin antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- Q13296
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